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Saving As You Go
It is a good practice to give your new protocol a title and save it immediately after choosing and opening a startup protocol (either blank or from a ProtoGenie library) and save it after every major step. Also, after every major work session, it is a good practice to make and save a copy of it as a backup protocol in case you make radical changes and then find that they were not what you thought you wanted.
Saving protocols should be distinguished from saving the Results (data) of the execution of protocols. At the completion of an execution of a protocol, you should click on SAVE in the top menu.